|
| Status |
Public on Aug 29, 2022 |
| Title |
4210_d |
| Sample type |
RNA |
| |
|
| Source name |
MUC4-KD2 647-V
|
| Organism |
Homo sapiens |
| Characteristics |
transfection: MUC4-KD2
cell line: 647-V cells
cell type: bladder cancer
|
| Treatment protocol |
Comparisons of miRNA in 647-V bladder cancer cell line following stable MUC4 knocked down by ShRNA transfection (2 assays, KD1 and KD2) and MOCK corresponding control cells.
|
| Growth protocol |
RPMI 10% FCS, L-Gln 2 mM, Peni/strepto.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
manufacturer's instructions
|
| |
|
| Hybridization protocol |
8*15K Agilent-019118 Human miRNA Microarrays were scanned on an Agilent G2505C scanner.
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version V.10.5.1.1).
|
| Data processing |
Agilent Feature Extraction Software (v 10.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Sep 09, 2019 |
| Last update date |
Aug 29, 2022 |
| Contact name |
frédéric leprêtre |
| E-mail(s) |
[email protected]
|
| Organization name |
Lille University
|
| Department |
CHU of Lille, CBP
|
| Lab |
plateforme de génomique fonctionnelle et structurale
|
| Street address |
Boulevard du Pr Leclercq
|
| City |
lille |
| ZIP/Postal code |
59037 |
| Country |
France |
| |
|
| Platform ID |
GPL7731 |
| Series (1) |
| GSE137130 |
MiRnome profiling of Human bladder cancer line invalidated for the MUC4 gene by ShRNA. |
|
