|
| Status |
Public on Sep 30, 2019 |
| Title |
ck18HL2 |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic tissue
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Hindlimb somatopleure
Stage: HH18
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested via lysis in Trizol followed by isolation using RNeasy micro columns.
Libraries were synthesized from 200ng total RNA using the stranded TruSeq kit (Illumina) followed by 15 cycles of amplification.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
RNA-seq reads were mapped using STAR to reference genomes (galGal4 or droNov1)
Quantification via FeatureCounts
Differential gene expression was determined using DESeq.
Genome_build: galGal4 for chick or droNov1 for emu
Supplementary_files_format_and_content: Tab delimited .txt files with transcript counts generated from featureCounts
|
| |
|
| Submission date |
Sep 03, 2019 |
| Last update date |
Sep 30, 2019 |
| Contact name |
John Young |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Lab |
Tabin
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL19787 |
| Series (2) |
| GSE136774 |
Attenuated FGF signaling underlies the forelimb heterochrony in the emu Dromaius novaehollandiae (RNA-seq data) |
| GSE136776 |
Attenuated FGF signaling underlies the forelimb heterochrony in the emu Dromaius novaehollandiae |
|
| Relations |
| BioSample |
SAMN12684303 |
| SRA |
SRX6793605 |
