age: 11y, when sample taken gender: Male race: Hispanic illness: Bacteremia, Osteomyelitis treatment: Anti-staphylococcal antibiotic pathogen: Staphylococcus aureus, methicillin-susceptible
Extracted molecule
total RNA
Extraction protocol
RNEasy Mini Kit
Label
Biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol
EukGE-WS2v5
Scan protocol
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 System.
Description
The subject was infected with Staphylococcus aureus, methicillin-susceptible.
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
