|
| Status |
Public on May 14, 2009 |
| Title |
Adipose undernutrition in utero and during suckling rep3 |
| Sample type |
RNA |
| |
|
| Source name |
epididymal fat
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
gender: Male
age: 3 weeks
group assignment: UU
tissue: epididymal fat
|
| Growth protocol |
In utero 50% global food restriction (calculated based on pregnancy-day matched controls), followed by either ad lib feeding or continued 50% food restriction during suckling.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from total RNA according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
15ug of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix GeneChip Mouse Genome 430A 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression from epididymal fat of 3 week old male mouse, in utero undernutrition, undernutrition during suckling (UU)
|
| Data processing |
Data were analyzed using Affymetrix GCOS v.1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
|
| |
|
| Submission date |
May 13, 2009 |
| Last update date |
May 13, 2009 |
| Contact name |
Mary Elizabeth Patti |
| E-mail(s) |
[email protected]
|
| Phone |
6177351966
|
| Fax |
6177322593
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Research Division
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE16083 |
Accelerated Postnatal Growth Increases Lipogenic Gene Expression and Adipocyte Size in Low-Birth Weight Mice |
|
