|
| Status |
Public on Sep 16, 2019 |
| Title |
Titanium dioxide and estrogen 062619AFE18 |
| Sample type |
RNA |
| |
|
| Source name |
J774 macrophages exposed to titanium dioxide and estrogen
|
| Organism |
Mus musculus |
| Characteristics |
cell line: J774
|
| Treatment protocol |
Macrophages were plated in 24 well-plate and exposed to 10 µg/well of particles and 2 µg/mL of estrogen for 24 hrs.
|
| Growth protocol |
Macrophages, murine cell line J774, were maintained in 100-mm Petri dishes in DMEM free of phenol red, supplemented with 10% FBS, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL) and 10 mM HEPES.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was conducted through a RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Standard Affymatrix label protocol
|
| |
|
| Hybridization protocol |
Standard Affymatrix hybridization protocol
|
| Scan protocol |
Standard Affymatrix scan protocol
|
| Description |
Titanium dioxide and estrogen
|
| Data processing |
RMA Express normalization and background
|
| |
|
| Submission date |
Aug 01, 2019 |
| Last update date |
Sep 17, 2019 |
| Contact name |
Alexey V Fedulov |
| E-mail(s) |
[email protected]
|
| Organization name |
Alpert Medical School of Brown University
|
| Department |
RIH / Surgical Research
|
| Lab |
Fedulov Lab
|
| Street address |
593 Eddy St.
|
| City |
Providence |
| State/province |
Rhode Island |
| ZIP/Postal code |
02903 |
| Country |
USA |
| |
|
| Platform ID |
GPL18233 |
| Series (1) |
|
