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Sample GSM399368 Query DataSets for GSM399368
Status Public on May 02, 2009
Title T.4int8+.Th#2
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4int 8+ TCRhi
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Slide Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4int8+.Th#2, Positive Selection, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.0364
10344622 271.1739
10344624 290.3375
10344633 782.1148
10344637 239.8841
10344653 19.5731
10344658 209.9368
10344674 11.063
10344679 49.2993
10344707 215.024
10344713 265.1766
10344723 136.2607
10344725 51.0821
10344741 2733.2431
10344743 26.7435
10344750 521.7004
10344772 24.8283
10344789 223.2393
10344797 416.5629
10344799 259.1201

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399368_EA07068_56652_MoGene_T.4int8+.Th_#2.cel.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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