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Sample GSM399363 Query DataSets for GSM399363
Status Public on May 02, 2009
Title T.4+8int.Th#2
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8int TCRhi
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Slide Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4+8int.Th#2, Positive Selection, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 18.1856
10344622 249.7509
10344624 288.0079
10344633 1133.3558
10344637 229.7096
10344653 22.6449
10344658 272.611
10344674 12.5988
10344679 47.1678
10344707 324.1616
10344713 138.6568
10344723 227.9491
10344725 41.9391
10344741 3051.4339
10344743 24.4699
10344750 621.6952
10344772 32.6252
10344789 257.2623
10344797 602.3324
10344799 255.2403

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399363_EA07068_56649_MoGene_T.4+8int.Th_#2.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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