|
| Status |
Public on Apr 20, 2009 |
| Title |
H1-ES_sample3 |
| Sample type |
RNA |
| |
|
| Source name |
H1_ES
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: embryonic stem (H1_ES)
|
| Biomaterial provider |
Cellular Dynamics
|
| Treatment protocol |
cells were supplied as pellets in trizol
|
| Growth protocol |
http://encodewiki.ucsc.edu/EncodeDCC/index.php/Cell_lines#Cell_phenotype_study
Human H1 human male ES cells cultured on TESR/Matrigel and split one to six using dispase. Karyotypically normal, 95% Oct4 positive, differentiable into mesodermal, endodermal and ectodermal lineages
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted from cell pellets using Trizol protocol
|
| Label |
biotin
|
| Label protocol |
1ug of total RNA was labeled according to Standard Affymetrix WT protocol for Exon 1.0 STarrays
|
| |
|
| Hybridization protocol |
Standard Affymetrix WT protocol for Exon 1.0 STarrays
|
| Scan protocol |
Standard Affymetrix WT protocol for Exon 1.0 STarrays
|
| Description |
cell pellet in Trizol
|
| Data processing |
Raw CEL files were processed using Affymetrix Expression Console Software using RMA-Core-DABG
pgf-file = HuEx-1_0-st-v2.r2.pgf
clf-file = HuEx-1_0-st-v2.r2.clf
bgp-file = HuEx-1_0-st-v2.r2.antigenomic.bgp
meta-ps-file = HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
|
| |
|
| Submission date |
Apr 20, 2009 |
| Last update date |
Apr 20, 2009 |
| Contact name |
Sridar V Chittur |
| E-mail(s) |
[email protected]
|
| Phone |
518-591-7215
|
| Organization name |
SUNY-University at Albany
|
| Department |
Center for Functional Genomics
|
| Lab |
Microarray Core
|
| Street address |
One Discovery Drive, CRC 342G
|
| City |
Rensselaer |
| State/province |
NY |
| ZIP/Postal code |
12144 |
| Country |
USA |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE14863 |
ENCODE H1_ES cell phenotyping study |
|
