|
| Status |
Public on Mar 20, 2020 |
| Title |
Pten(het)-Lkb1(null)_Late carcinoma_4 |
| Sample type |
RNA |
| |
|
| Source name |
Late prostate tumor tissue from a Ptenpc+/- Lkb1pc-/- mouse
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Ptenpc+/- Lkb1pc-/-
tumor type: Late prostate tumor tissue
tissue: Prostate tumor tissue (FFPE)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from FFPE tissue using RNAstorm FFPE RNA extraction kit (Cell Data Sciences) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
cRNA is converted to biotinylated single-stranded cDNA prepared according to the standard Affymetrix protocol from 12 ngs total RNA (GeneChip Whole Trasncript WT Expression Arrays, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5,5 ug of fragmented and labeled ss-cDNA were hybridized for 16 hr at 45ºC at 60 rpm on Hybridization Oven 640 de Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 7G de Affymetrix
|
| Data processing |
RMA processing
|
| |
|
| Submission date |
Jul 04, 2019 |
| Last update date |
Mar 20, 2020 |
| Contact name |
Cristian Suarez Cabrera |
| E-mail(s) |
[email protected]
|
| Phone |
+34 699044222
|
| Organization name |
CIEMAT
|
| Lab |
Molecular Oncology Unit
|
| Street address |
Av. Complutense 40
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
| GSE133837 |
Complete LKB1 loss results in lethal metastatic prostate cancer |
|
