|
| Status |
Public on Apr 14, 2009 |
| Title |
Arabidopsis Columbia in control medium biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
columbia plants in control medium
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: root
ecotype: Col-0
genotype: wild type
|
| Treatment protocol |
Plats were grown for on control media for 10 days and then transferred to either control or phosphate-free media for 10 h.
|
| Growth protocol |
Plants were grown on agar medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Qiagen RNeasy mini kit.
|
| Label |
biotin
|
| Label protocol |
Ten microgram of total RNA was used for cDNA synthesis, labeled by in vitro transcription, followed by fragmentation according to the manufacturer's suggestion (GeneChip Expression Analysis Technical Manual rev5, Affymetrix)
|
| |
|
| Hybridization protocol |
Ten microgram labeled samples were hybridized to the ATH1 Genome Array at 45℃ for 16.5 h. Washing and staining involved Fluidic Station-450 and the ATH1 Genome.
|
| Scan protocol |
Array was scanned with use of the Affymetrix GeneChip Scanner 7G.
|
| Description |
gene expression data from arabidopsis roots under iron deficiency medium
|
| Data processing |
The results were quantified and analyzed by use of MicroArray Suite 5.0 software (Affymetrix Inc.).
|
| |
|
| Submission date |
Apr 13, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Wolfgang Schmidt |
| E-mail(s) |
[email protected]
|
| Phone |
+886-2-2787-1162
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Plant and Microbial Biology
|
| Street address |
No. 128, Sec 2, Academia Rd, Nankang
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE15649 |
Early phosphate-deficiency induced signal changes in Arabidopsis roots |
|
| Relations |
| Reanalyzed by |
GSE119083 |
