|
| Status |
Public on Aug 21, 2009 |
| Title |
AGMBR_Blood_biological replicate II 2000018 |
| Sample type |
RNA |
| |
|
| Source name |
blood
|
| Organism |
Chlorocebus sabaeus |
| Characteristics |
ucla animal id: 2000-018
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from brain tissues with PefectPure RNA Cell and Tissue Kit (5 Prime) and from peripheral blood collected to PaxGene RNA blood tubes using PAXgene Blood RNA Kit (PreAnalyticX).
|
| Label |
biotin
|
| Label protocol |
cDNA was synthesized and in vitro transcribed into biotinylated cRNA using the Illumina Totalprep RNA amplification kit, following the manufacturer’s instructions (Ambion)
|
| |
|
| Hybridization protocol |
Illumina Beadstation, standard Illumina protocol
|
| Scan protocol |
Illumina Beadstation, standard Illumina protocol
|
| Description |
Illumina_project_number: 2008-063
|
| Data processing |
Raw signal was background corrected in BeadStudio. VST transformation and RSN normalization was performed using Lumi package.
|
| |
|
| Submission date |
Apr 08, 2009 |
| Last update date |
Aug 21, 2009 |
| Contact name |
Anna J Jasinska |
| Organization name |
University of Pittsburgh
|
| Department |
School of Medicine
|
| Street address |
3550 Terrace Street
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15261 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE15301 |
Identification of brain transcriptional variability reproduced in peripheral blood: an approach for mapping brain eQTLs |
|
