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Status
Public on Jan 10, 2005
Title
ANOXIA+SUCROSE EXPERIMENT 1
Sample type
RNA
Source name
4 days old, dark grown seedlings
Organism
Arabidopsis thaliana Extracted molecule
total RNA
Description
Arabidopsis thaliana ecotype Columbia glabra were grown for 4 days in the dark at 23°C in multiwell plates containing half-strenght murashige skoog medium without added sucrose. Sample was subsequently kept for 6h under anoxia in the presence of 90mM sucrose. RNA was extracted using a Ambion RNaqueous kit. Targets were produced using standard Affymetrix procedures from about 10ug of total RNA. Each independent experiment consisted of four replicated seedlings cultures, pooled after RNA extraction.
Submission date
Jan 07, 2005
Last update date
Aug 28, 2018
Contact name
Pierdomenico Perata
E-mail(s)
[email protected] Phone
+39050881901
Organization name
Sant'Anna School of Advanced Studies
Department
Institute of Life Sciences
Lab
PlantLab
Street address
Via Guidiccioni 8-10
City
San Giuliano Terme
State/province
Pisa
ZIP/Postal code
56010
Country
Italy
Platform ID
GPL198 Series (1)
GSE2133 Effects of anoxia and sucrose on seedling growth
Relations Reanalyzed by
GSE119083
Data table header descriptions ID_REF
VALUE 'signal' a measure of the abundance of a transcript (not scaled)
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
119.7
P
0.00011
AFFX-BioB-M_at
171.6
P
0.000044
AFFX-BioB-3_at
115
P
0.000044
AFFX-BioC-5_at
271.2
P
0.000044
AFFX-BioC-3_at
140.2
P
0.000044
AFFX-BioDn-5_at
290.9
P
0.000044
AFFX-BioDn-3_at
1078.9
P
0.000044
AFFX-CreX-5_at
2833.5
P
0.000044
AFFX-CreX-3_at
3550.6
P
0.000044
AFFX-DapX-5_at
1.5
A
0.440646
AFFX-DapX-M_at
4.5
A
0.52976
AFFX-DapX-3_at
0.3
A
0.984817
AFFX-LysX-5_at
4.5
A
0.15671
AFFX-LysX-M_at
5.2
A
0.645547
AFFX-LysX-3_at
0.4
A
0.574038
AFFX-PheX-5_at
0.3
A
0.986189
AFFX-PheX-M_at
0.5
A
0.932322
AFFX-PheX-3_at
2.6
A
0.60308
AFFX-ThrX-5_at
1.2
A
0.814869
AFFX-ThrX-M_at
0.6
A
0.868639
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