|
| Status |
Public on Dec 31, 2019 |
| Title |
B6D2F1_beta-cell_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreatic beta-cell from B6D2F1, 2days after EMC-D virus infection, replicate3
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6D2F1
tissue: Pancretic beta-cell
Sex: male
treatment: 48hr after EMC-D virus infection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The cRNA was amplified, labeled using GeneChip WT PLUS Reagent Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Affymetrix Clariom D Assay Mouse array, washed and stained according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Affymetrix scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).
|
| Data processing |
The data were analyzed with median polish summarization and quantile normalization by Affymetrix® Expression Console™ 1.1 software, and obtained normalized intensity values.
|
| |
|
| Submission date |
Jun 07, 2019 |
| Last update date |
Jan 01, 2020 |
| Contact name |
Keiichiro Mine |
| E-mail(s) |
[email protected]
|
| Organization name |
Saga university
|
| Street address |
5-1-1 Nabeshima
|
| City |
Saga City |
| State/province |
Saga |
| ZIP/Postal code |
849-0937 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
| GSE132388 |
Gene expression in pancreatic beta-cells post EMC-D virus infection |
|
