|
| Status |
Public on Nov 04, 2019 |
| Title |
EE_scRNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
FACS sorted Enteroendocrine cell
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: intestine
age: adult stage 5-7 days old
genotype: CG32547-KI-GAL4>UAS-GFP
|
| Growth protocol |
Flies were cultured on standard media with yeast paste added to the food surface, and the culture temperature was 25℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA and library was prepared by following the manufacturer’s instructions of 10X genomics scRNA-seq
Libraries were prepared for sequencing using standard Illumina protocols
10X genomics single cell RNA-sequencing
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
G101_1_TACTCT_L003
G101_2_CCTGTG_L003
G101_3_GGACAC_L003
G101_4_ATGAGA_L003
|
| Data processing |
cellranger V2.0
Genome_build: BDGP6
Supplementary_files_format_and_content: cellranger V2.0 outputs
|
| |
|
| Submission date |
Jun 06, 2019 |
| Last update date |
Nov 05, 2019 |
| Contact name |
xi rongwen |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Biological Sciences, Beijing
|
| Street address |
No. 7 Science Park Road, zhongguancun Life Science Park, Changping District,
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL23702 |
| Series (1) |
| GSE132274 |
Single-cell sequencing of enteroendocrine cells (EEs) in Drosophila midgut. |
|
| Relations |
| BioSample |
SAMN11964706 |
| SRA |
SRX5979983 |
