|
| Status |
Public on Jun 04, 2020 |
| Title |
RNAseq_St5_controlRNAi_male_embryos_02 |
| Sample type |
SRA |
| |
|
| Source name |
blastoderm embryos, nc14
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: progeny of mat-a-tubGal4/controlRNAi mothers crossed to their siblings (Bloomington stocks: 7062 & 36304)
tissue: blastoderm embryos, nc14
Sex: male
|
| Growth protocol |
D. melanogaster flies were maintained in standard Drosophila medium at 25oC.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Embryos of the desired staged were identified under the stereoscope. Single embryos were homogenized in Trizol and total mRNA was isolated using the microRNA Zymo Kit. RNA Quality was assesed by Fragment Analyzer.
Truseq Stranded mRNA
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
St5_RNAseq_counts.txt
St5_control_male_02
single embryo
|
| Data processing |
quality control,mapping and count retrieval was done as described in Bhardwaj, V. et al. "snakePipes enable flexible, scalable and integrative epigenomic analysis." Biorxiv 407312 (2018). doi:10.1101/407312
Genome_build: dm6
Supplementary_files_format_and_content: gene counts
|
| |
|
| Submission date |
Apr 25, 2019 |
| Last update date |
Jun 04, 2020 |
| Contact name |
Asifa Akhtar |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute of Immunobiology and Epigenetics
|
| Department |
Chromatin Regulation
|
| Lab |
Akhtar Lab
|
| Street address |
Stuebeweg 51
|
| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23323 |
| Series (2) |
| GSE130333 |
H4K16ac developmental dynamics during Drosophila development [RNA] |
| GSE130335 |
Intergenerationally maintained Histone H4 lysine 16 acetylation is instructive for future gene activation |
|
| Relations |
| BioSample |
SAMN11507312 |
| SRA |
SRX5736511 |
