|
| Status |
Public on Mar 23, 2019 |
| Title |
bladder_cancer_turbt_sample_50 [GNDX-MGH-QCFCG50] |
| Sample type |
RNA |
| |
|
| Source name |
FFPE bladder cancer
|
| Organism |
Homo sapiens |
| Characteristics |
age: NA
Sex: Male
cstage: T2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After pathologic review, tissue was harvested from formalin-fixed, paraffin-embedded tumor blocks by 1mm punch under RNAse-free conditions. Total RNA was extracted and purified using RNeasy FFPE kit (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
RNA was amplified, fragmented and biotinylated using the WT-Ovation FFPE system (NuGen, San Carlos, CA) according to the manufacturer’s recommendations with minor modifications
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials and protocols
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Data was normalized uisng SCAN (SCAN.UPC package from Bioconductor)
probe group file: HuEx-1_0-st-v2.r2.pgf
meta-probeset file: HuEx-1_0-st-v2.r2.dt1.hg38.core.mps
|
| |
|
| Submission date |
Mar 21, 2019 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Elai Davicioni |
| E-mail(s) |
[email protected]
|
| Organization name |
GenomeDx
|
| Street address |
1152 Mainland St #430
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6B 4X2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL22995 |
| Series (1) |
| GSE128701 |
Impact of Immune and Stromal Infiltration on Outcomes Following Bladder-Sparing Trimodality Therapy for Muscle-Invasive Bladder Cancer |
|
