|
| Status |
Public on Mar 01, 2019 |
| Title |
C1_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
control_blood RNA
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: control
tissue: whole blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from whole blood collected in PAXgene Blood RNA Tubes using PAXgene Blood RNA Kit (PreAnalytiX) per manufacturer’s instructions
Total RNA was used for cDNA library preparation using the TruSeq Stranded mRNA Library Preparation kit for NeoPrep (Illumina). Sequencing was performed on an Illumina HiSeq 3000 System in a 1 x 50 bp single read mode
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
RNA-seq reads were mapped against GRCh38 using hisat 2.2.1.0. Mapped reads were quantified using HTSeq. All the count data were normalized by total mapped reads.
Genome_build: GRCh38
Supplementary_files_format_and_content: Read-count text file
|
| |
|
| Submission date |
Feb 28, 2019 |
| Last update date |
Mar 03, 2019 |
| Contact name |
Daniel L Kastner |
| E-mail(s) |
[email protected]
|
| Organization name |
NIH
|
| Department |
NHGRI
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
| GSE127572 |
Transcriptomic profiling of cleavage-resistant RIPK1 induced autoinflammation syndrome patients |
|
| Relations |
| BioSample |
SAMN11039032 |
| SRA |
SRX5446161 |
