|
| Status |
Public on Feb 14, 2020 |
| Title |
Young PDGFRa+ mesenchymal progenitors |
| Sample type |
RNA |
| |
|
| Source name |
PDGFRa+ cells from young hind limb, freshly sorted
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
age: Young (8-10 wk)
cell type: PDGFRa+ mesenchymal progenitors
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using RNeasy micro kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl aRNA was synthesized by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion #1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc.
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shaking (250 rpm). Hybridization buffer and washing protocol were followed by the protocol supplied by TORAY Industries, Inc.
|
| Scan protocol |
3D-Gene Scanner (Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction (Toray Industries Inc., Tokyo, Japan).
|
| Description |
Y Pα
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Feb 16, 2019 |
| Last update date |
Feb 15, 2020 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL22438 |
| Series (2) |
| GSE126665 |
Gene expression analysis of cell populations derived from skeletal muscle of young and aged mice |
| GSE126667 |
Gene expression analysis of skeletal muscle |
|
