|
| Status |
Public on Nov 10, 2009 |
| Title |
393P, sample a1173 |
| Sample type |
RNA |
| |
|
| Source name |
393P
|
| Organism |
Mus musculus |
| Characteristics |
393P (non-metastatic)
|
| Growth protocol |
Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
|
| Description |
393P (non-metastatic), sample a1173
|
| Data processing |
The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
|
| |
|
| Submission date |
Jan 15, 2009 |
| Last update date |
Nov 10, 2009 |
| Contact name |
Chad Creighton |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE14458 |
Gene expression profiles of 344SQ lung adenocarcinoma cells with high metastatic potential (syngeneic mouse model) |
| GSE14459 |
NSCLC metastasis: K-ras/p53 mutant and syngeneic mouse models |
|
