|
| Status |
Public on Apr 15, 2009 |
| Title |
Mouse 3, Sample:SZ24, Sex:M, Primary lung cancer, Adenocarcinoma |
| Sample type |
RNA |
| |
|
| Source name |
Primary lung adenocarcinoma from p53R172H∆g/+ K-rasLA1/+ mice
|
| Organism |
Mus musculus |
| Characteristics |
Mouse3, Sample:SZ24, Sex:M, Primary lung cancer, Adenocarcinoma
|
| Growth protocol |
Primary lung adenocarcinomas and metastases from p53R172H∆g/+ K-rasLA1/+ mice or syngeneic tumors were isolated, carefully dissected to remove the adjacent tissue.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol (Invitrogen) and purified with an RNeasy kit (Qiagen). RNA quality and quantity were evaluated on an Agilent Bioanalyzer following the manufacture’s recommendations (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on Mouse430A GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
|
| Description |
Mouse3, Sample:SZ24, Sex:M, Primary lung cancer, Adenocarcinoma
|
| Data processing |
The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
|
| |
|
| Submission date |
Jan 15, 2009 |
| Last update date |
Mar 31, 2009 |
| Contact name |
Chad Creighton |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (2) |
| GSE14449 |
Gene expression profiles of spontaneous metastasis in a K-ras/p53 mutant mouse model |
| GSE14459 |
NSCLC metastasis: K-ras/p53 mutant and syngeneic mouse models |
|
