|
| Status |
Public on Feb 14, 2019 |
| Title |
M5_90_2 from RAW264.7 cells infected with B. melitensis M5-90 for 4h |
| Sample type |
RNA |
| |
|
| Source name |
M5_90_2
|
| Organism |
Mus musculus |
| Characteristics |
cell line: RAW264.7 cells
infection condition: B. melitensis M5-90
infection time: 4h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Animal Tissue RNA Purification Kit (Norgen Biotek Corporation, Thorold, Canada), following the manufacturer's recommended protocol.
|
| Label |
cy3
|
| Label protocol |
The assay started from 2 to 5 μg total RNA sample were 3’-extended with a poly(A) tail using poly(A) polymerase. An oligonucleotide tag was then ligated to the poly(A) tail for later fluorescent dye staining.
|
| |
|
| Hybridization protocol |
Hybridization was performed overnight on a μParaflo microfluidic chip using a micro-circulation pump (Atactic Technologies).
|
| Scan protocol |
Hybridization images were collected using a laser scanner (GenePix 4000B, Molecular Device) and digitized using Array-Pro image analysis software (Media Cybernetics).
|
| Description |
Fourteen differentially expressed miRNAs were found in the two groups
|
| Data processing |
Data were analyzed by first subtracting the background and then normalizing the signals using a LOWESS filter (Locally-weighted Regression).
|
| |
|
| Submission date |
Feb 13, 2019 |
| Last update date |
Feb 14, 2019 |
| Contact name |
Xie qin |
| E-mail(s) |
[email protected]
|
| Phone |
15618926050
|
| Organization name |
LC sciences
|
| Street address |
Minchi first road, pujiang town, minhang district, Shanghai
|
| City |
shanghai |
| ZIP/Postal code |
200000 |
| Country |
China |
| |
|
| Platform ID |
GPL20871 |
| Series (2) |
| GSE126498 |
The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by b. melitensis m5-90 [miRNA] |
| GSE126499 |
The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by B. melitensis m5-90 |
|
