|
| Status |
Public on Sep 30, 2019 |
| Title |
WT4_1ng |
| Sample type |
SRA |
| |
|
| Source name |
Frontal Cortex
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Frontal Cortex
strain: C57BL/6
age: 3 month post-natal
genotype: WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
3-month-old GFAP-IL6 homozygous or wild-type mice were euthanized by cervical dislocation followed by decapitation. Frontal cortex was dissected and snap-frozen in RNAse-free tubes on dry ice. Total RNA was isolated by Trizol-chloroform extraction, followed by RNeasy clean-up.
3'Pool-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
the indicated amound of RNA (_ng) was added to the Reverse Transcription reaction.
|
| Data processing |
aligned to hg19 reference genome using STAR v2.4
count uniquely aligned reads per gene using FeatureCount from Subread package and gencode v19 as the gene model annotation
|
| |
|
| Submission date |
Jan 24, 2019 |
| Last update date |
Sep 30, 2019 |
| Contact name |
Hualin Xi |
| E-mail(s) |
[email protected]
|
| Organization name |
AbbVie
|
| Street address |
34 webb st
|
| City |
Lexington |
| State/province |
Massachusetts |
| ZIP/Postal code |
02420 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE125569 |
3’Pool-seq: an optimized cost-efficient and scalable method of whole-transcriptome gene expression profiling (Run0064) |
| GSE125571 |
3’Pool-seq: an optimized cost-efficient and scalable method of whole-transcriptome gene expression profiling |
|
| Relations |
| BioSample |
SAMN10799584 |
| SRA |
SRX5286758 |
