|
Status |
Public on Jan 01, 2019 |
Title |
bladder_cancer_RC_sample_85 |
Sample type |
RNA |
|
|
Source name |
FFPE bladder cancer
|
Organism |
Homo sapiens |
Characteristics |
pstage: pT2 gender: Male cluster: CC4_Scar-like tissue: bladder cancer RC sample
|
Treatment protocol |
Not Applicable
|
Growth protocol |
Not Applicable
|
Extracted molecule |
total RNA |
Extraction protocol |
After pathologic review, tissue was harvested from formalin-fixed, paraffin-embedded tumor blocks by 1mm punch under RNAse-free conditions. Total RNA was extracted and purified using RNeasy FFPE kit (Qiagen, Valencia, CA)
|
Label |
biotin
|
Label protocol |
RNA was amplified, fragmented and biotinylated using the WT-Ovation FFPE system (NuGen, San Carlos, CA) according to the manufacturer’s recommendations with minor modifications
|
|
|
Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials and protocols
|
Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
Description |
GDXPostNAC85
|
Data processing |
Data was normalized uisng SCAN (SCAN.UPC package from Bioconductor) HuEx-1_0-st-v2.r2.pgf HuEx-1_0-st-v2.r2.dt1.hg38.core.mps
|
|
|
Submission date |
Dec 22, 2018 |
Last update date |
Jan 14, 2019 |
Contact name |
Elai Davicioni |
E-mail(s) |
[email protected]
|
Organization name |
GenomeDx
|
Street address |
1152 Mainland St #430
|
City |
Vancouver |
State/province |
BC |
ZIP/Postal code |
V6B 4X2 |
Country |
Canada |
|
|
Platform ID |
GPL22995 |
Series (1) |
GSE124305 |
Divergent biological response to neoadjuvant chemotherapy in muscle-invasive bladder cancer |
|