|
| Status |
Public on Dec 18, 2008 |
| Title |
U266 myeloma cells-treted_DMSO_4h |
| Sample type |
RNA |
| |
|
| Source name |
U266 myeloma cells-treted_DMSO_4h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: multiple myeloma cell line
t(11;14)
|
| Treatment protocol |
Cells were treated with DMSO 0.1% (+/- pristimerin 500nM dissolved in DMSO) for 4h at 37oC
|
| Growth protocol |
Myeloma cell lines were cultured in RPMI 10% FBS at 37oC in 5%CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16h at 45 degrees Celsius onto Affymetrix GeneChip arrays with rotisserie mixing at 60rpm. GeneChips were washed and stained with streptavidin-phycoerythrin.
|
| Scan protocol |
GeneChips were scanned using the GeneChip system confocal scanner.
|
| Description |
U266 myeloma cells were treated with DMSO 0.1% for 4h at 37oC and total RNA was prepared using Trizol
|
| Data processing |
The data were PLIER16 transformed using Affymetrix Expression Console and Excel and paired with detection calls generated from Affymetrix Expression ConsoleMAS5 analysis using default analysis settings
|
| |
|
| Submission date |
Dec 17, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
rodger tiedemann |
| E-mail(s) |
[email protected]
|
| Organization name |
Mayo Clinic
|
| Department |
Division of Hematology Oncology
|
| Street address |
13400 E Sahuaro Dr
|
| City |
Scottsdale |
| State/province |
AZ |
| ZIP/Postal code |
85259 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14011 |
Expression data from myeloma cell lines treated with 500 nM pristimerin or DMSO vehicle alone for 4 hours |
|
| Relations |
| Reanalyzed by |
GSE119087 |
