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Sample GSM3510286 Query DataSets for GSM3510286
Status Public on Jun 10, 2019
Title presurgical patient 003 [granulocytes]
Sample type RNA
 
Source name peripheral granulocytes from presurgical patient
Organism Homo sapiens
Characteristics diagnosis: presurgical
cell type: peripheral granulocytes
Treatment protocol Granulocytes were prepared from 2 mL blood by positive selection (StraightFrom™ Whole Blood CD15 MicroBeads, Miltenyi Biotec).
Growth protocol Blood was collected in S-Monovette® K3E tubes (Sarstedt, Nümbrecht, Germany) by venipuncture with presurgical patients and from a central venous catheter with ICU patients.
Extracted molecule total RNA
Extraction protocol Total RNA was purified from isolated granulocytes stored in RNAlater® (Ambion) using the miRVana miRNA isolation kit (Applied Biosystems).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000.
Description S_B_003
Gene expression data from peripheral granulocytes of presurgical patient.
Data processing The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of human Hugene-2_0-type from Affymetrix. A Custom CDF Version 20 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
 
Submission date Dec 12, 2018
Last update date Jun 10, 2019
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL21970
Series (2)
GSE123729 AKIRIN1 is a suitable reference gene in natural killer cells and granulocytes from patients with SIRS and septic shock [granulocytes]
GSE123731 AKIRIN1 is a suitable reference gene in natural killer cells and granulocytes from patients with SIRS and septic shock

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100009613_at 3.197265625
100009676_at 4.524414063
10000_at 3.183105469
10001_at 6.056640625
10002_at 3.376953125
100033411_at 2.745117188
100033413_at 3.164550781
100033422_at 2.859863281
100033423_at 2.8203125
100033424_at 2.563964844
100033425_at 2.262207031
100033426_at 8.138671875
100033427_at 9.919921875
100033428_at 2.317382813
100033430_at 2.354003906
100033431_at 5.15234375
100033432_at 2.4140625
100033434_at 2.358398438
100033435_at 8.919921875
100033436_at 2.558105469

Total number of rows: 24733

Table truncated, full table size 511 Kbytes.




Supplementary file Size Download File type/resource
GSM3510286_Lindner_230516_S-B-003_HuGene-2_0-st_.CEL.gz 7.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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