|
| Status |
Public on Apr 15, 2020 |
| Title |
RA EPC-derived endothelial cells - hypoxia - 2 |
| Sample type |
RNA |
| |
|
| Source name |
EC derived from circulating EPCs_RA_hypoxic exposure
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: Rheumatoid arthritis
tissue: peripheral blood
cell type: Endothelial cells (EC) derived from circulating endothelial progenitor cells (EPCs)
treatment: hypoxic exposure
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA with RNeasy Mini kit with DNase I digestion according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100ng of total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA were hybridized for 16 hr at 45C on GeneChip HumanExon1.0ST and chips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Description |
Gene expression from RA-derived endothelial cells - hypoxia
A5_(HuEx-1_0-st-v2).CEL
|
| Data processing |
The data were RMA normalized with Bioconductor using Version18 of Brain Array Human Exon1.0 CDF file.
|
| |
|
| Submission date |
Oct 29, 2018 |
| Last update date |
Apr 16, 2020 |
| Contact name |
Franck Letourneur |
| E-mail(s) |
[email protected]
|
| Organization name |
INSERM
|
| Department |
U1016
|
| Lab |
genomic
|
| Street address |
22 rue mechain
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL25734 |
| Series (1) |
| GSE121894 |
Gene expression profile of endothelial cells derived from circulating progenitors issued from patients with rheumatoid arthritis |
|
