|
| Status |
Public on Apr 16, 2020 |
| Title |
MCF7_R2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
cell type: breast cancer cell line
patient age: old
|
| Growth protocol |
Cell lines were cultured in RPMI 1640 medium supplemented with 1% L-glutamine and 10% fetal bovine serum (GIBCO, New York, NY). The culture conditions were identical in all cell lines: 37 ̊C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from BC cell lines was isolated in passage 4 using High Pure RNA Isolation Kit from Roche following the manufacturer’s protocol
|
| Label |
biotin
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
Hybridization and scanning were performed according to the Affymetrix standard protocol, using Affymetrix Expression Console software.
|
| Scan protocol |
Hybridization and scanning were performed according to the Affymetrix standard protocol, using Affymetrix Expression Console software.
|
| Data processing |
Data quality of *.CEL sample files was analyzed by affy package from R/Bioconductor
|
| |
|
| Submission date |
Oct 17, 2018 |
| Last update date |
Apr 17, 2020 |
| Contact name |
Sara OLTRA SANCHIS |
| E-mail(s) |
[email protected]
|
| Phone |
961973540
|
| Organization name |
INCLIVA
|
| Department |
Medical Oncology
|
| Lab |
Cancer Functional Genomics
|
| Street address |
Avda Menéndez Pelayo, 4 accesorio
|
| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46010 |
| Country |
Spain |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE121396 |
miRNA expression in breast cancer cell lines |
|
