|
| Status |
Public on Feb 10, 2011 |
| Title |
no relapse _ sample 148 |
| Sample type |
RNA |
| |
|
| Source name |
no relapse
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: white blood cells
class: pediatric acute leukemia
tissue: bone marrow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
White blood cells were isolated through Ficoll-Hypaque density gradient centrifugation. Lysis of the mononuclear cells followed by lysate homonogenization using a biopolymer shredding system in a microcentrifuge spin-column format followed by total RNA purification using selective binding columns.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450DX.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000Dx.
|
| Description |
Gene expression data from pediatric acute leukemia samples at diagnosis
|
| Data processing |
The data were normalized using RMA algorithm in R software [www.r-ptoject.org], BioConductor package [www.bioconductor.org]
|
| |
|
| Submission date |
Nov 13, 2008 |
| Last update date |
May 04, 2011 |
| Contact name |
Andrea Zangrando |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Padova
|
| Department |
Woman and Child's Health
|
| Lab |
Hemato-Oncology
|
| Street address |
Via Giustiniani, 3
|
| City |
Padova |
| ZIP/Postal code |
35138 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13576 |
Early Relapse in ALL is identified by Time To Leukemia in NOD/SCID mice and is characterized by a gene signature involving survival pathways |
|
