|
| Status |
Public on Sep 17, 2020 |
| Title |
Gastrocnemius_24m_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Gastrocnemius_24m
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
Sex: female
age: 24 month
tissue: Gastrocnemius
|
| Treatment protocol |
After reaching the age of 24 (~6 month) or 103 weeks (~24 month), mice were sacrificed after overnight fasting.
|
| Growth protocol |
Female C57BL/6J mice were group housed and received standard chow (#2018, containing 18% protein, 50% carbohydrate and 6.0% fat; Harlan Laboratories, Madison, WI, USA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were frozen in liquid nitrogen for biochemical and molecular analyses. Total RNA was isolated using Trizol (Life Technologies) and purified using the RNeasy Mini Kit (Qiagen) in accordance with the manufacturer’s instructions. It was then assessed for degradation using an Advanced Analytical Agilent Fragment Analyzer.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA was amplified using Ambion® WT Expression Kit from life technologies (Part Number 4411974) according to manufactures protocols, then 5500ng cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit (part number 900671) according to manufactures protocols.
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized according to Affymetrix protocols. Approximately 16hr incubation and then washed and stained according to Affymetrix protocols on Affymetrix 450 Fluidics Station.
|
| Scan protocol |
Arrays were scanned on Affymetrix GSC3000 7G Scanner
|
| Description |
G_O_3
|
| Data processing |
Microarray data was normalized with RMA-sketch method and analyzed using the Affymetrix Expression and Transcriptome analysis consoles.
|
| |
|
| Submission date |
Sep 21, 2018 |
| Last update date |
Sep 18, 2020 |
| Contact name |
Xu Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
École polytechnique fédérale de Lausanne
|
| Department |
School of Life Sciences
|
| Lab |
IBI-SV NCEM
|
| Street address |
EPFL SV IBI-SV NCEM AI 1148 (Bâtiment AI) Station 15
|
| City |
Lausanne |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
| GSE120290 |
Transcriptome analysis of metabolic tissues in young and aged mice |
|
