|
| Status |
Public on Dec 26, 2018 |
| Title |
MT_297 |
| Sample type |
SRA |
| |
|
| Source name |
mFUS-/-/hgFUS R521C
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: mFUS-/-/hgFUS R521C
tissue: Spinal cord
age: 18 months
strain: C57Bl/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol from 18-month-old spinal cords. RNA quality was measured using the Agilent Bioanalyzer system and processed using the Illumina TruSeq Stranded mRNA Sample Preparation Kit according to manufacturer’s protocols.
A total of 14 cDNA libraries were simultaneously generated using llumina TruSeq Stranded mRNA Sample Preparation Kit and sequenced using an Illumina HiSeq 2000 sequencer.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
MT_297
|
| Data processing |
fastqs were aligned to a mouse reference genome (mm9, UCSC Genome Browser) by TopHat and the transcript abundance for each annotated protein-coding gene as fragments per kilobase of transcript per million mapped reads (FPKM) were estimated by Cufflinks.
Genome_build: mm9
Supplementary_files_format_and_content: FPKM
|
| |
|
| Submission date |
Sep 20, 2018 |
| Last update date |
Dec 26, 2018 |
| Contact name |
Sandrine Da Cruz |
| E-mail(s) |
[email protected]
|
| Phone |
8585344299
|
| Organization name |
Ludwig Institute for Cancer Research-UCSD
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
Ca |
| ZIP/Postal code |
92093-0670 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE120247 |
ALS/FTD-linked mutation in FUS suppresses intra-axonal protein synthesis and drives disease without nuclear loss-of-function of FUS |
|
| Relations |
| BioSample |
SAMN10096621 |
| SRA |
SRX4721105 |
