|
| Status |
Public on Dec 26, 2018 |
| Title |
GCB DLBCL patient_RR12013196 |
| Sample type |
RNA |
| |
|
| Source name |
Patient n°12R13196, frozen GCB DLBCL biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: GCB DLBCL biopsy (lymph node)
|
| Growth protocol |
No culture was performed, frozen tissues were used for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA from frozen samples was performed according to the manufacturer's instructions. Post extraction, RNA were purified with clean-up and DNAse I (Rneasy mini kit, Qiagen). RNA quantification was performed on a Nanodrop (ND-1000). RNA quality was controlled with RNA 6000 Nano chip on a Bioanalyzer 2100 (Agilent).
|
| Label |
biotin
|
| Label protocol |
RNAs were amplified, prepared and labelled from 100 pg total RNA, with the GeneCHIP® WT PLUS Reagent Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip® Human Transcriptome Array (HTA) 2.0 microarrays.
|
| Scan protocol |
After hybridization, arrays were washed and stained with streptavidin-phycoerythrin (GeneChip Hybridization Wash and Stain Kit) in a fluidic 450 (Affymetrix). The arrays were scanned with the Genechip scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from patient 12R13196, frozen GCB DLBCL biopsy
|
| Data processing |
The data were analyzed on Expression Console version 1.4.1 (Affymetrix) with "RMA gene full" normalization using default analysis settings.
|
| |
|
| Submission date |
Sep 18, 2018 |
| Last update date |
Dec 26, 2018 |
| Contact name |
Pauline Gravelle |
| E-mail(s) |
[email protected]
|
| Organization name |
IUCT-ONCOPOLE
|
| Department |
Anatomy-Pathology
|
| Street address |
1 Avenue Irène Joliot Curie
|
| City |
TOULOUSE |
| ZIP/Postal code |
31059 |
| Country |
France |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE120124 |
Expression data from 40 lymphomas (Hodgkin, B cell, T cell) |
|
