|
| Status |
Public on Aug 21, 2009 |
| Title |
WT_Saline_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Lung tissues from littermate control mice challenged with saline
|
| Organism |
Mus musculus |
| Characteristics |
C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNEasy columns. RNA integrity was confirmed using an Agilent 2100 Bioanalyzer
|
| Label |
Cy3
|
| Label protocol |
400ng of RNA was amplified and labeled using the One-Color Agilent Low RNA Input Linear Amplification Kit
|
| |
|
| Hybridization protocol |
Labeled cRNA was assessed using the Nandrop ND-100 , and equal amounts of Cy3 labeled target were hybridized to Agilent whole genome custom arrays (platform acc# GPL7202). Hybridizations were performed for 14 hrs, according to the manufacturers protocol.
|
| Scan protocol |
Arrays were scanned using the Agilent microarray scanner
|
| Description |
11 arrays were hybridized and represent 3 lung samples for groups WT saline, WT OVA and KO OVA. There are 2 samples for the KO saline group.
|
| Data processing |
Raw signal intensities were extracted with Feature Extraction v9.1 software (Agilent). The dataset was normalized using the quantile normalization method. The median feature pixel intensity was used as the raw signal before normalization and no background subtraction was performed.
|
| |
|
| Submission date |
Oct 28, 2008 |
| Last update date |
Aug 21, 2009 |
| Contact name |
Andrea J Barczak |
| Organization name |
UCSF Sandler Center Functional Genomics Core Facility
|
| Street address |
1550 4th St Rock Hall 545
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE13382 |
Distinct roles of FOXA2 and FOXA3 in allergic airway disease |
|
