|
| Status |
Public on Oct 08, 2019 |
| Title |
Osteogenic medium and PBS, Rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Osteogenic medium and PBS, BMSCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Mesenchymal stem cells
culture: Osteogenic medium
stimulation: PBS
|
| Treatment protocol |
Human bone marrow MSCs were treated with osteogenic medium and PBS (Control)/perivascular stem cell-derived extracellular vesicles (2.5 μg/mL) for 48 hours
|
| Growth protocol |
Mesenchymal stem cells (MSCs) were isolated from human bone marrow and cultured in DMEM medium plus 15% FBS, 1% pen/strep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell monolayers were lysed with Invitrogen Life Technologies TRIzol Reagent (Total RNA Isolation Reagent) and precipitated using the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled with the Affymetrix GeneChip WT PLUS Reagent Kit using the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
17 hours at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual.
|
| Scan protocol |
Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual.
|
| Description |
OM4
|
| Data processing |
Affymetrix CEL files were extracted with the Partek GS 7.0 platform and their data RMA and quantile-normalized to create log2 transcript signal values, which were used in subsequent ANOVA analysis.
Clariom_D_Human.r1.pgf
Clariom_D_Human.na36.hg38.probeset.csv
|
| |
|
| Submission date |
Aug 23, 2018 |
| Last update date |
Oct 08, 2019 |
| Contact name |
Aaron W James, MD, PhD |
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Pathology
|
| Lab |
Ross 524
|
| Street address |
600 North Wolfe Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE118961 |
Expression data from human bone marrow mesenchymal stem cells - treated with perivascular stem cell-derived extracellular vesicles control (in osteogenic medium) |
|
