|
| Status |
Public on Oct 17, 2008 |
| Title |
Lens from Pax6 heterozygote rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA of eye lens of newborn Pax6-heterozygous mouse
|
| Organism |
Mus musculus |
| Characteristics |
Pax6 heterozygous RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Amplified cDNA was fragmented and biotin labeled according to manufacturer's instructions (NuGEN Ovation ™ Biotin RNA Amplification and Labeling System ).
|
| |
|
| Hybridization protocol |
2.2 micrograms of cDNA was hybridized for 17 hr to the Affymetrix array as described in the Ovation™ Biotin RNA Amplification and Labeling System manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.
|
| Scan protocol |
Affymetrix GeneArray scanner 7G
|
| Description |
Lenses were dissected from newborn Pax6 heterozygous and wildtype pups.
|
| Data processing |
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
|
| |
|
| Submission date |
Oct 16, 2008 |
| Last update date |
Mar 18, 2009 |
| Contact name |
Jiri ZAVADIL |
| E-mail(s) |
[email protected]
|
| Phone |
+33648065733
|
| Organization name |
IARC WHO
|
| Street address |
150 COURS ALBERT THOMAS
|
| City |
LYON |
| State/province |
Other |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE13244 |
Identification of Pax6-dependent gene regulatory networks in the mouse lens |
|
