|
| Status |
Public on Oct 18, 2008 |
| Title |
fetal_liver_hematopoietic_stem_cells |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
200319 |
| Tag Length |
17 |
| |
|
| Source name |
fetal liver, FACS sorted Lin-/Mac1+/CD43+/Sca1+
|
| Organism |
Mus musculus |
| Characteristics |
Strain : Pep3B
Tissue: Fetal Liver
Age: E14.5
FACS sorted: Lin-/Mac1+/CD43+/Sca1+
Purity: 95%
Number of cells: 20000
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Starting RNA: ~10ng (24 cycles of PCR used)
Isolation method: Arcturus PicoPure [total RNA was extracted using the PicoPure kit (Arcturus), a column based method that isolates polyA RNA (http://www.moleculardevices.com/pages/reagents/picopure.html)]
Storage Medium: H2O
DNaseI treatment: Yes
Method of RNA (cDNA) quant: Agilent
SAGE type: PCR-LongSAGE
|
| |
|
| Description |
Murine fetal liver was harvested and stained as described in Bowie et al. PNAS 2007 and the cells Lin-/Mac1+/CD43+/Sca1+ were sorted on a FACSVantage. Individual single cell transplantation experiments into sublethally irradiated recipients confirm that the HSC purity is at ~1 in 10.
|
| |
|
| Submission date |
Oct 15, 2008 |
| Last update date |
Nov 19, 2008 |
| Contact name |
BCGSC BC Cancer Agency |
| E-mail(s) |
[email protected]
|
| Organization name |
Canada's Michael Smith Genome Sciences Centre
|
| Department |
Gene Expression
|
| Lab |
Marco Marra
|
| Street address |
675 West 10th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1349 |
| Series (1) |
| GSE13243 |
SAGE Libraries from Highly Purified Mouse Hematopoietic Stem Cells from the E14.5 Fetal Liver and Adult Bone Marrow |
|
