|
| Status |
Public on Jan 01, 2019 |
| Title |
H_Control-4: Primary hepatocyte, control, rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Primary hepatocyte, control
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Primary hepatocyte
|
| Treatment protocol |
Primary hepatocytes were treated with 0.1% DMSO (control) or 200 nM FK866 (NAMPT inhibitor) for 48 hours. Primary islets were treated with 0.1% DMSO or 10 nM FK866 for 48 hours.
|
| Growth protocol |
Primary hepatocyte or pancreatic islets were isolated from wild type B6 mice and cultured.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from primary hepatocytes and pacreatic islets treated with DMSO or FK866 using Rneasy Mini Kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 4
|
| Data processing |
The background-subtracted raw microarray data were also normalized by Z score transformation to obtain Z ratios. All data were analyzed by the R statistical software package.
|
| |
|
| Submission date |
Aug 09, 2018 |
| Last update date |
Sep 15, 2023 |
| Contact name |
Jun Yoshino |
| E-mail(s) |
[email protected]
|
| Organization name |
Faculty of Medicine, Shimane University
|
| Street address |
89-1 Enya-cho
|
| City |
Izumo |
| State/province |
Shimane |
| ZIP/Postal code |
693-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15097 |
| Series (1) |
| GSE118365 |
Slc12a8 is a nicotinamide mononucleotide transporter that counteracts aging |
|
