|
| Status |
Public on Mar 20, 2019 |
| Title |
Ga13ΔM/ΔM_RANKL |
| Sample type |
SRA |
| |
|
| Source name |
Bone Marrow Macrophages
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: Bone Marrow Macrophages
genotype: Ga13flox/floxLysMcre(+)
agent: RANKL
|
| Treatment protocol |
Bone marrow macropahges were treated with RANKL for 5days.
|
| Growth protocol |
Bone marrow was flushed from femur and tibia and cultured with M-CSF for 3 days to get bone marrow macrophages.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using RLT buffer from Rneasy RNA extraction kit. mRNA was purified by poly-A+ selection. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using STAR
Transcripts Per Million(TPM) were calculated using edgeR.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each Sample
|
| |
|
| Submission date |
Jul 02, 2018 |
| Last update date |
Mar 20, 2019 |
| Contact name |
Kazuki Inoue |
| Organization name |
HOSPITAL FOR SPECIAL SURGERY
|
| Street address |
535 E70TH STREET, S building 8th floor
|
| City |
NEW YORK |
| State/province |
New York |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE116550 |
Ga13 functions as a cytoskeletal and mitochondrial regulator to restrain osteoclast function |
|
| Relations |
| BioSample |
SAMN09534813 |
| SRA |
SRX4335303 |
