|
| Status |
Public on Aug 01, 2018 |
| Title |
RanBP9D1 /BSC469_3 |
| Sample type |
SRA |
| |
|
| Source name |
ovaries
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: RanBP9D1 /BSC469
replicate: 3
|
| Growth protocol |
All flies were maintained at +25°C.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted from dissected ovaries with TRIzol (15596026, Thermofisher) according to the manufacturer’s protocol
NEBNext Ultra II Directional RNA Library after purification with polyA binding beads
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
data sets were aligned using TopHat2 Drosophila genome dm6
Counting aligned reads per genes were performed with HTSeq
Differential expression analysis was performed with DESeq2
Genome_build: dm6 (BDGP6.87)
Supplementary_files_format_and_content: text file of expression values per sample in RPKM format
|
| |
|
| Submission date |
Jun 27, 2018 |
| Last update date |
Aug 01, 2018 |
| Contact name |
Maria Sokolova |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Helsinki
|
| Department |
Institute of Biotechnology
|
| Lab |
Nuclear Actin Lab
|
| Street address |
Viikinkaari 5
|
| City |
Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL19132 |
| Series (2) |
| GSE116363 |
Nuclear actin is required for transcription during Drosophila oogenesis [RNA-seq] |
| GSE116365 |
Nuclear actin is required for transcription during Drosophila oogenesis |
|
| Relations |
| BioSample |
SAMN09505332 |
| SRA |
SRX4315091 |
