|
| Status |
Public on Jun 27, 2018 |
| Title |
PAT17 |
| Sample type |
genomic |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
case status: case
phenotype: KS
mutation: None
Sex: M
slide id: 8655677134
array position: R05C01
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA (1125 ng) was prepared, for each sample, in a total volume of 45 μl and bisulfite treated using the EZ DNA Methylation kit (Zymo Research Corp, Orange, CA, 114 USA), as specified by the manufacturer for use with the Infinium Human Methylation 450 BeadChip arrays
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Standard Illumina Protocol
|
| Scan protocol |
Standard Illumina Protocol
|
| Data processing |
removed poorly performing probes (detection p value > 0.01); performed quantile normalization using the minfi package in R-3.1.2
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Jun 26, 2018 |
| Last update date |
Jun 27, 2018 |
| Contact name |
Hans Tomas Bjornsson |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
McKusick-Nathans Institute of Genetic Medicine
|
| Street address |
733 N. Broadway
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE116300 |
Patients with a Kabuki syndrome phenotype demonstrate DNA methylation abnormalities. |
|
