|
| Status |
Public on Jun 01, 2020 |
| Title |
Patient4_corifollitrophin alfa |
| Sample type |
RNA |
| |
|
| Source name |
hGCs_cori-a_24h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Primary culture of human granulosa cells (hGCs)
|
| Treatment protocol |
hGCs primary cultures were maintained in starvation buffer for 12 hours. hGCs were treated with bioequivalent doses of corifollitrophin alfa or rec FSH. hGCs were evaluated after 24 hours from the removal of the unbound gonadotrophins.
|
| Growth protocol |
hGCs were recovered from preovulatory ovarian follicles after the oocytes retrieval from the medium aspirated. Retrieved hGCs were cultured for 6 days in McCoy 5A medium (Carlo Erba, Italy) supplemented with 5% foetal bovine serum (FBS) South America (EU Approved, Carlo Erba, Italy), 2mM L-Glutamine, 1% Penicillin/Streptomicin and 1% Amphotericin B (Sigma Aldrich, St. Louis, MO, USA) at 37 °C under a controlled atmosphere of 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
not provided
|
| Scan protocol |
not provided
|
| Data processing |
affymetrix-algorithm-name = sst-rma-gene-full
program-name = Expression Console
program-version = 1.4.1.46
|
| |
|
| Submission date |
Jun 15, 2018 |
| Last update date |
Jun 01, 2020 |
| Contact name |
Antonio La Marca |
| E-mail(s) |
[email protected]
|
| Phone |
+390594224671
|
| Organization name |
University of Modena and Reggio
|
| Department |
Mother-Infant Department, Institute of Obstetrics and Gynecology
|
| Street address |
Via del pozzo, 71
|
| City |
Modena |
| State/province |
Modena |
| ZIP/Postal code |
41100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE115846 |
Gene Expression Profiles of Human Granulosa Cells Treated With Bioequivalent Doses of Corifollitrophin Alfa or Recombinant Human Follicle-Stimulating Hormone |
|
