|
Status |
Public on Jun 01, 2019 |
Title |
5410 D vs. 6866 D |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
5410
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype: Rspo3++ cell type: colon cells cell sorting: colon distal
|
Treatment protocol |
Tamoxifen (Sigma) was injected intraperitoneally into mice either as single dose or on three consecutive days (4 mg/25 g bodyweight, diluted in 200 µl corn oil) at the indicated time points to deplete Rspo3 expression
|
Growth protocol |
Animals were maintained in autoclaved microisolator cages and provided with sterile drinking water and chow ad libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated by the Trizol (Invitrogen) method according the manufacturer´s protocol.
|
Label |
Cy3
|
Label protocol |
RNA labeling was performed with the Quick-Amp Labeling Kit (Agilent Technologies) according supplier's instructions.
|
|
|
Channel 2 |
Source name |
6866
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype: Rspo3 KO cell type: colon cell sorting: colon distal
|
Treatment protocol |
Tamoxifen (Sigma) was injected intraperitoneally into mice either as single dose or on three consecutive days (4 mg/25 g bodyweight, diluted in 200 µl corn oil) at the indicated time points to deplete Rspo3 expression
|
Growth protocol |
Animals were maintained in autoclaved microisolator cages and provided with sterile drinking water and chow ad libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated by the Trizol (Invitrogen) method according the manufacturer´s protocol.
|
Label |
Cy5
|
Label protocol |
RNA labeling was performed with the Quick-Amp Labeling Kit (Agilent Technologies) according supplier's instructions.
|
|
|
|
Hybridization protocol |
Whole mouse genome 4x44k microarrays were done according to the supplier’s protocol (Agilent Technologies).
|
Scan protocol |
Scanning of microarrays was performed with 5 µm resolution and extended range (XDR) using a DNA microarray laser scanner (Agilent Technologies)
|
Description |
polarity(+) 251486841825_1_1
|
Data processing |
Raw microarray image data were analyzed with the Image Analysis / Feature Extraction software G2567AA (Version A.11.5.1.1, Agilent). The extracted MAGE-ML files were analyzed with the Rosetta Resolver Biosoftware (Rosetta Biosoftware). Intra-array data were normalized with the GE2_1105_Oct12 extraction protocol and interarray normalization was done by the mean of trimmed positive non-flagged/non-control reporters.
|
|
|
Submission date |
Jun 13, 2018 |
Last update date |
Jun 01, 2019 |
Contact name |
Hans-Joachim Mollenkopf |
E-mail(s) |
[email protected]
|
Phone |
+49 30 28460 482
|
Organization name |
Max-Planck-Institute for Infection Biology
|
Lab |
Microarray/Genomics Core Facility
|
Street address |
Charitéplatz 1
|
City |
Berlin |
ZIP/Postal code |
10117 |
Country |
Germany |
|
|
Platform ID |
GPL4134 |
Series (1) |
GSE115752 |
R-spondin 3 regulates stem cell plasticity and recovery of damaged colon epithelium |
|