|
| Status |
Public on Nov 30, 2018 |
| Title |
NHA KO 1 |
| Sample type |
genomic |
| |
|
| Source name |
brain astrocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: astrocytes
genotype: IDH3a KO
tissue: brain
|
| Treatment protocol |
No treatments were applied
|
| Growth protocol |
NHA Control and KO cells were grown to confluency in 15 cm dishes. They were then trypsinized, counted, and collected.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
gDNA was then extracted using the Blood & Cell Culture DNA Mini Kit from Qiagen following the manufacturer's protocol
|
| Label |
Cy5 and Cy3
|
| Label protocol |
standard Illumina Protocol
|
| |
|
| Hybridization protocol |
standard Illumina Protocol
|
| Scan protocol |
standard Illumina Protocol
|
| Description |
immortalized cell line
|
| Data processing |
R software (version 3.4.3) Bioconductor package minfi
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Jun 05, 2018 |
| Last update date |
Nov 30, 2018 |
| Contact name |
Yanrong Ji |
| E-mail(s) |
[email protected]
|
| Organization name |
Northwestern University
|
| Department |
Feinberg School of Medicine
|
| Lab |
Davuluri Lab
|
| Street address |
Arthur J. Rubloff Building, 750 North Lake Shore Drive, 11th Floor
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE115382 |
IDH3a KO effect on global DNA methylation |
|
