|
| Status |
Public on May 11, 2021 |
| Title |
Hdac3-/- 50d rep2 |
| Sample type |
SRA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Alb-Cre:Hdac3-/-
age: 50 day after sPH (2-3 mohths-old)
|
| Treatment protocol |
Liver tissues were collected at defined points
|
| Growth protocol |
WT liver(WT1-WT3) and Alb-Cre:Hdac3−/− liver (0d(Ha-Hc), 7d(Hd-Hg), 21d(Hh-Hk), 50d(Hl-Hn)) receiving successive PH(sPH), Alb-Cre:Hdac3−/− tumor tissues(HCC1-HCC3) and adjacent non-tumor tissues(N1-N3), spontaneous liver cancers of Alb-Cre:Hdac3-/- mice (HCC1-HCC3) and liver tumor of recipient Fah-/- mice (HCCa-HCCc)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Livers were collected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
The filtered data was compared with reference sequence by software HISAT and Bowtie2.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample.
|
| |
|
| Submission date |
May 15, 2018 |
| Last update date |
May 11, 2021 |
| Contact name |
ji hong jie |
| E-mail(s) |
[email protected]
|
| Organization name |
West China Hospital
|
| Street address |
37 Guoxue Road, Chengdu, China
|
| City |
chengdou |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL23479 |
| Series (1) |
| GSE114503 |
Next Generation Sequencing Facilitates Quantitative Analysis of three groups from mouse liver Transcriptomes |
|
| Relations |
| BioSample |
SAMN09213271 |
| SRA |
SRX4088684 |
