|
| Status |
Public on Sep 01, 2008 |
| Title |
18Q_1 |
| Sample type |
RNA |
| |
|
| Source name |
Primary rat striatal neuron cultures, 4 weeks in vitro
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sample group: 18Q; Tissue: Lentivirus-infected primary neuronal culture from rat striatum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini system (Qiagen, Hilden, Germany) followed by ethanol precipitation
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip® IVT Labeling Kit, according to manufacturer's protocol (GeneChip® Expression Analysis Technical Manual 701021 Rev. 5)
|
| |
|
| Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol (GeneChip® Expression Analysis Technical Manual 701021 Rev. 5)
|
| Scan protocol |
Affymetrix® GeneChip® Scanner 3000 with GCOS software according to manufactuer's protocol (GeneChip® Expression Analysis Technical Manual 701021 Rev. 5)
|
| Description |
Infected 4 weeks prior with TRE-Htt-N853-18Q-expressing recombinant lentivirus
|
| Data processing |
Probe set summarization and normalization was performed by robust multi-array averaging (RMA, including background subtraction, summarization by median polish and quantile normalization) using the R/Bioconductor package affy.
|
| |
|
| Submission date |
Aug 19, 2008 |
| Last update date |
Aug 19, 2008 |
| Contact name |
Alexandre Kuhn |
| E-mail(s) |
[email protected]
|
| Organization name |
NIH
|
| Department |
NIA
|
| Lab |
Laboratory of Neurogenetics
|
| Street address |
35 Convent Dr
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-3707 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE12481 |
TRE-Htt-N853 Huntington's Disease in vitro model |
|
