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Sample GSM312016 Query DataSets for GSM312016
Status Public on Jul 25, 2009
Title Right atria dnPI3K 1648
Sample type RNA
 
Source name RNA from mouse heart atria, dnPI3K
Organism Mus musculus
Characteristics male C57BL/6/FVB/N
Treatment protocol Transgenics models dnPI3k, Mst1 and dnPI3K-Mst1 were made accordingly.
Growth protocol The mice were on a standard chow diet, and were kept in temperature controlled cages (21 - 23 degree celcius) and maintained a 12 hour light/dark cycle
Extracted molecule total RNA
Extraction protocol Heart was homogenized individually using motar and pestle in liquid nitrogen. Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen).
Label biotin
Label protocol Fragmented and biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA for Affymetrix Mouse Gene 1.0 ST arrays
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were processed through second-cycle single stranded cDNA, fragmented and labelled cDNA were then hybridized for 16 hr at 45C on Affymetrix Mouse Gene 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Gene arrays were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from heart atria
Data processing Gene array data is processd by Expression Console software in which mean probeset signals for all probesets are analysed. A receiver operating curve (ROC) is generated by thrseholding the computed value for the probesets. PLIER signals that pass this QC control is used for subsequent analysis.
 
Submission date Aug 12, 2008
Last update date Jul 24, 2009
Contact name Ruby CY Lin
E-mail(s) [email protected], [email protected]
Organization name The Westmead Institute for Medical Research
Street address 176 Hawkesbury Road, Westmead
City Sydney
State/province NSW
ZIP/Postal code 2145
Country Australia
 
Platform ID GPL6246
Series (1)
GSE12420 Gene profiling of heart atria in PI3K and Mst1 mouse models

Data table header descriptions
ID_REF
VALUE Expression Console signal intensity (in Log2 space).

Data table
ID_REF VALUE
10338001 11.691800117493
10338003 10.448100090027
10338004 9.3388996124268
10338017 12.510899543762
10338025 9.0307197570801
10338026 12.939399719238
10338029 9.4946002960205
10338035 9.0573501586914
10338036 9.8087997436523
10338037 4.6804399490356
10338041 10.727299690247
10338042 10.210499763489
10338044 11.815600395203
10338047 8.1191701889038
10338056 2.8718800544739
10338059 12.928199768066
10338060 5.4237899780273
10338063 3.8238699436188
10338064 7.166130065918
10338065 8.1733903884888

Total number of rows: 35512

Table truncated, full table size 863 Kbytes.




Supplementary file Size Download File type/resource
GSM312016.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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