|
| Status |
Public on Mar 06, 2019 |
| Title |
thyroid tumor, total thyroidectomy, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
thyroid tumor tissue_liquid nitrogen snap-frozen immediately
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: thyroid tumor tissue
pathologic diagnosis: papillary thyroid carcinoma
gender: female
age: 26
t stage (cm): 3.3*1.7
n stage: N1a
m stage: M0
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and RNA integrity was determined by 1% formaldehyde denaturing gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
The miRNAs were labeled using the Agilent miRNA labeling reagent. Total RNA (200 ng) was dephosphorylated and ligated with pCp-Cy3, the labeled RNA was purified and hybridized to miRNA arrays.
|
| |
|
| Hybridization protocol |
According to the Agilent miRNA Microarray Protocol (Agilent Technologies).
|
| Scan protocol |
After hybridization and washes, slides were scanned immediately in Agilent Microarray Scanner with Surescan High Resolution Technology (Agilent Technologies, Santa Clara, CA).
|
| Description |
microRNA expression profile
|
| Data processing |
Feature Extraction v10.10 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and Bioconductor software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Apr 25, 2018 |
| Last update date |
Mar 06, 2019 |
| Contact name |
Xia Fada |
| E-mail(s) |
[email protected]
|
| Organization name |
Central South university
|
| Street address |
NO. 87, Xiangya Road
|
| City |
Changsha, China |
| ZIP/Postal code |
410000 |
| Country |
China |
| |
|
| Platform ID |
GPL24741 |
| Series (1) |
| GSE113629 |
microRNA expression profiling of papillary thyroid carcinoma (PTC) and matched adjacent normal thyroid tissues |
|
