|
| Status |
Public on Oct 31, 2008 |
| Title |
Protected, d5 |
| Sample type |
RNA |
| |
|
| Source name |
Laser-capture microdissected islets from NOD.Scid injected at d0 with BDC2.5 transgenic, diabetogenic T cells and also injected twice with anti-Ifng antibodies
|
| Organism |
Mus musculus |
| Characteristics |
pooled from 5 NOD.Scid mice protected from developing diabetes by Ifng antibody injection
|
| Biomaterial provider |
Emil Unanue and Jason C. Mills
|
| Treatment protocol |
Captured RNA was extracted using Agilent PicoPure columns, pooled and ~30 ng was amplified using one round of Arcturus RiboAmp HS kit amplification followed by the RNA Amplification and Labeling Kit from Enzo Life Sciences
|
| Growth protocol |
mice were sacrificed 5 days after transfer of CD4+ BDC2.5 diabetogenic T cells and following two injections of anti-Ifng antibody (at day -1 and day 2 relative to T cell transfer)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Islets were captured from frozen sections that had been fixed in methanol, briefly eosin-stained in ethanol and immediately dehydrated without exposure to aqueous solution at any time. Agilent PicoPure column with DNAse treatment was used to extract RNA from captured laser-capture caps.
|
| Label |
biotin and phycoerythrin
|
| Label protocol |
Affymetrix-recommended cRNA synthesis with biotinylated nucleotides followed by fragmentation
|
| |
|
| Hybridization protocol |
Affymetrix standard
|
| Scan protocol |
Affymetrix standard
|
| Description |
Protected, d5
|
| Data processing |
dChip used to normalize and model all chips in this series
|
| |
|
| Submission date |
Aug 06, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jason C Mills |
| E-mail(s) |
[email protected]
|
| Phone |
7137984607
|
| Organization name |
Baylor College of Medicine
|
| Department |
Gastroenterology, Medicine
|
| Street address |
One Baylor Plaza
|
| City |
Houston. |
| State/province |
T |
| ZIP/Postal code |
77401 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE12389 |
Interferon-γ-dependent regulatory circuits in immune inflammation highlighted in diabetes |
|
| Relations |
| Reanalyzed by |
GSE119085 |
