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Sample GSM309501 Query DataSets for GSM309501
Status Public on Nov 04, 2008
Title immature-B3
Sample type RNA
 
Source name B220+ CD43- IgM+ IgD-
Organism Mus musculus
Characteristics cell type: normal immature B
C57Bl6/129
Extracted molecule total RNA
Extraction protocol Trizol extraction following manufacturer's protocol.
Label Biotin
Label protocol 20 nanograms of total RNA was amplified and processed to produce biotinylated cDNA targets using the NuGen Ovation Biotin labeling system.
 
Hybridization protocol 3.75 micrograms of biotinylated material was hybridized according to the standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol
Description purified immature B-lymphocytes (B220+ CD43- IgM+ IgD-) from adult mouse bone marrow
Data processing RMA background correction and quantile normalization done with the GenePattern ExpressionFileCreator module (http://www.broad.mit.edu/cancer/software/genepattern/) which uses the Bioconductor implementation of RMA (http://www.bioconductor.org/). This function computes the Robust Multichip Average expression measure described in Irizarry et al Biostatistics (2003).
 
Submission date Jul 31, 2008
Last update date Mar 18, 2009
Contact name Madeleine E. Lemieux
E-mail(s) [email protected]
Phone 617-595-6695
URL http://www.bioinfo.ca
Organization name Bioinfo
Street address 275 Main St., Suite 252
City Plantagenet
State/province ON
ZIP/Postal code K0B 1L0
Country Canada
 
Platform ID GPL8321
Series (2)
GSE12310 Expression profiling of normal murine lymphoid progenitors and MLL-AF4 leukemic lymphoblasts
GSE12363 H3K79 methylation profiles define murine and human MLL-AF4 leukemias

Data table header descriptions
ID_REF
VALUE RMA-normalized intensity

Data table
ID_REF VALUE
1415670_at 651.4496727
1415671_at 1093.840225
1415672_at 1191.660996
1415673_at 236.2340341
1415674_a_at 536.8285942
1415675_at 1011.773118
1415676_a_at 1780.042051
1415677_at 399.9222549
1415678_at 3108.434181
1415679_at 1755.915298
1415680_at 684.2760515
1415681_at 1183.890628
1415682_at 349.4413814
1415683_at 908.3378481
1415684_at 390.2150425
1415685_at 468.4903303
1415686_at 1605.726123
1415687_a_at 5109.633349
1415688_at 1044.16647
1415689_s_at 205.4835848

Total number of rows: 22690

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM309501.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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