|
| Status |
Public on Oct 01, 2018 |
| Title |
MI-FTC_normal_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Non-tumorous region, MI-FTC, FFPE
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Thyroid
gender: female
age: 50
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the NucleoSpin® totalRNA FFPE XS Kit (MACHEREY-NAGEL, Düren, Germany) according to the manufacturer's instructions. On-column DNaseI digestion was performed to avoid genomic DNA contamination.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared usibg the GeneChip® WT Pico Reagent Kit from 100 ng total RNA (GeneChip® WT Pico Reagent Kit User Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Fifteen ug of cRNA were subjected to fragmentation using the 3' fragmentation buffer hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
RNA from FFPE specimens
|
| Data processing |
The data were analyzed with Affymetrix Transcriptome Analysis Console® using RMA as a summerization algorithm.
|
| |
|
| Submission date |
Mar 06, 2018 |
| Last update date |
Oct 01, 2018 |
| Contact name |
OSAMU ISHIBASHI |
| E-mail(s) |
[email protected]
|
| Phone |
+81-72-254-9474
|
| Organization name |
Osaka Prefecture University
|
| Department |
Grad Sch Life & Environmental Sciences
|
| Lab |
Biological Macromolecules
|
| Street address |
1-1 Gakuen-cho, Naka-ku
|
| City |
Sakai |
| ZIP/Postal code |
599-8531 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE111455 |
Array-based genome-wide transcriptome analysis of Minimally invasive follicular thyroid carcinomas |
|
