|
| Status |
Public on May 22, 2019 |
| Title |
HV_003 |
| Sample type |
RNA |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
condition: Healthy Control
|
| Treatment protocol |
Freshly drawn peripheral blood collected in PAXgene tubes from healthy volunteers and patients with confirmed Marfan syndrome
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA including miRNAs of all MFS patients and HV controls was isolated with the PAXgene miRNA blood kit using the QIAcube™ automated isolation instrument
|
| Label |
Cyanine 3-PCP
|
| Label protocol |
100 ng total RNA from each sample was processed using the miRNA Complete Labeling and Hyb Kit (Agilent) to generate fluorescently labeled miRNA. The labeled RNA was then applied to the microarray slide per the manufacturer's instructions and hybridized in a rotating oven for 20 hours at 55°C and 20 rpm.
|
| |
|
| Hybridization protocol |
standard Agilent protocol
|
| Scan protocol |
Arrays were washed and then scanned using a DNA Microarray Scanner (Agilent). Feature extraction software was utilized to extract gene expression data (Agilent).
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Feb 21, 2018 |
| Last update date |
May 22, 2019 |
| Contact name |
Masood Abu-Halima |
| E-mail(s) |
[email protected]
|
| Organization name |
Univeristy of Saarland
|
| Department |
Human Genetics
|
| Lab |
1.06
|
| Street address |
Kirrberger St 60
|
| City |
Homburg Saar |
| ZIP/Postal code |
66421 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16770 |
| Series (2) |
| GSE110965 |
Deregulated MicroRNA and mRNA Expression Profiles in the Peripheral Blood of Patients with Marfan Syndrome (microRNA) |
| GSE110966 |
Deregulated MicroRNA and mRNA Expression Profiles in the Peripheral Blood of Patients with Marfan Syndrome |
|
